Bupropion monograph using word template.doc

Monograph produced on behalf of LTG for the purpose of public education in analytical toxicology Bupropion
Background (e.g. history, therapeutic use, abuse, effects):
Historically, bupropion (amfebutamone) has been available in the U.S. as an antidepressant (Wellbutrin®). It is structurally unrelated to other antidepressants but has a therapeutic efficacy similar to tricyclic antidepressants. However, it has recently been prescribed in the U.K. as a smoking cessation adjunct (Zyban®). It is not prescribed in the U.K. as an antidepressant. There have been no reported instances of abuse of the drug in the U.K. but it is structurally similar to the phenylethylamines and therefore may result in various analytical problems e.g. false positive amphetamine results using immunoassay. Although the neurochemical effect of bupropion is not completely understood, there is evidence that it may block dopamine re-uptake and block serotonin and noradenaline uptake. It does not appear to inhibit monoamine oxidase. Chemical Data
Chemical name:
Chemical formula and weight:
239.74 (weight)
CAS number:
Metabolism and Pharmacokinetics
Bupropion is extensively metabolised by hydroxylation and reduction to form 3 less active major metabolites; (I) morpholinol metabolite (hydroxybupropion) (II) erythroamino alcohol metabolite and (III) threoamino alcohol metabolite. The 3 metabolites all have longer half-lives than the parent drug and less than 0.5% of a single dose is excreted unchanged in urine. The plasma half-life appears to be approximately 14 hours. There is also some published data regarding storage of bupropion stored in plasma at high temperatures (half-life at 22oC = 54 hours, at 37oC = 11 hours). There appeared to be no effect on the stability of the major metabolites. Monograph produced on behalf of LTG for the purpose of public education in analytical toxicology
Toxicity (including non-fatal and fatal data)
There are limited published data regarding toxicity and resulting blood
concentrations, however, it has been reported that following ingestion of a single 100
mg dose, an in life peak plasma bupropion concentration of 0.14 mg/L was attained.
In overdose, toxic effects have been noted to be anxiety, tremor and tachycardia. In
five U.S. cases of fatalities involving bupropion, post mortem blood concentrations of
4.0 mg/L (unspecified site), 0.16 mg/L (unspecified site), 4.2 mg/L (unspecified site),
11.0 mg/L (femoral) and 13.0 mg/L (subclavian). In one U.K. case of death involving
suspected bupropion overdose a post mortem blood concentration of 5.7 mg/L
(femoral) was measured.
Proprietary preparations
Wellbutrin (USA)
Zyban (UK)
In the U.K. bupropion is usually prescribed initially at 150 mg three times a day,
followed by a reduction to 150 mg twice a day.
Zyban (bupropion HCl)
Size: Diameter (mm) = not specified
Monograph produced on behalf of LTG for the purpose of public education in analytical toxicology
Analytical Data
GC-MS mass-spectrum
Bupropion (underivatised)
LC-MS mass-spectrum
Not available
GC elution data
Elutes between illicit drugs BDB and MBDB on DB-5 column. Parent bupropion and
all metabolites are separated.

HPLC elution data
OD/CN column = co-elution of parent compound and one metabolite (MeCN and
water 0-70% ramp)
“Silica column” (e.g. ODS) = separation of parent and metabolites

UV spectrum
Monograph produced on behalf of LTG for the purpose of public education in analytical toxicology Immunoassay data
EMIT amphetamine assay = ?cross-reaction
CEDIA amphetamine assay = >18 mg/L in urine gives positive result

Monograph produced by
Simon Elliott, Regional Laboratory for Toxicology, City Hospital NHS Trust, Birmingham, U.K.

Martindale – The Extra Pharmacopoeia, 30th Ed. Ed. James Reynolds,
Pharmaceutical Press, 1993, page 250.
R. C. Baselt, "Bupropion" in Disposition of toxic drugs and chemicals in man (5th
Ed.), 2000.
P. N. Friel et al, “Three fatal drug overdoses involving bupropion”, J. Anal. Tox., vol
17, 1993, pages 436-438.
T. A. Jennison et al, “A HPLC method for quantitating bupropion in human plasma
and serum”, J. Anal. Tox., vol 19, 1995, pages 69-72.
T. A. Rohrig and N. G. Ray, “Tissue distribution of bupropion in a fatal overdose”, J.
Anal. Tox., vol 16, 1992, pages 343-345.
S. P. Elliott, unpublished findings in a fatality involving bupropion, 2001.
S. C. Laizure and C. L. DeVane, “Stability of bupropion and its major metabolites in
human plasma”, Ther. Drug Monit., vol 7, 1985, pages 447-450.
N. Wohlenberg et al, “Postmortem quantitation of bupropion (Wellbutrin) in blood
using the Finnigan Ion Trap Detector”, TIAFT Bulletin, vol 22 (4), 1992.
J. E. Meeker et al, “A suicidal overdose of bupropion (Wellbutrin)”, TIAFT Bulletin, vol
22 (4), 1992.

Source: http://homepixel-ru.1gb.ru/bibl/Bupropion.pdf

Microsoft word - morosycristianosbayarque.doc

Historia y tradiciones de Bayarque Anexo I Relaciones de Moros y Cristianos de Bayarque 1 Historia y tradiciones de Bayarque Relación de la conquista de Bayarque, del robo sacrílego de los Santos Patronos y de la Reconquista de los Cristianos. Fiestas Patronales. Primer Domingo de Octubre. Bayarque INDICE I. De la


CERTIFICATO DI CONFORMITA' Disciplinare ECO BIO COSMESI PRODUCT CERTIFICATE ECO BIO COSMETICS Standard Nome dell'operatore - Name of the operator Per verificare la validità del certificato visita il sito WWW.ICEA.INFO - You can check the validity of this certificate on the webs D.T.R 06 e Reg. RC COSM D.T.R 06 e Reg. RC COSM Ufficio di emissione - Au

© 2010-2017 Pdf Pills Composition