02-cairns- atul

Asian J. Exp. Sci., Vol. 22, No. 1, 2008; 143-146 Concurrent Effects of Eyestalk Ablation and Fluoxetine on the
Nutrient Depostion During Ovarian Development in a Fresh Water
Prawn, Machrobrachium lamarrei lamarrei
R. Eswaralakshmi, J. Jayanthi and M.G. Ragunathan
Department of Advanced Zoology and Biotechnology,
Guru Nanak College,
Abstract : The organic compounds like protein, carbohydrate and lipid have been quantitatively
estimated in tissues such as haemolymph, hepatopancreas, muscle and ovary after injecting
fluoxetine to an eyestalk ablated female fresh water prawn, Macrobrachium lamarrei lamarrei
and all the tissues show fluctuations in their contents due to fluoxetine treatment. The results
show that the more deposition of organic contents in the ovary of the fluoxetine treated prawn
due to stimulatory action of fluoxetine.
Key words : Bilateral eyestalk ablation, prawns, hepatopancreas, muscle, haemolymph, ovary,
Biochemical contents
Introduction
fluoxetine has not done in an eyestalk ablated process, in which biochemical composition of the animals occurs. The hepatopancreas is an important site of intermediary metabolism Materials and Methods
source of vitellogenin precursors. There is an Macrobrachium lamarrei lamarrei in the present study were collected from a lake in hormones in some crustaceans may regulate the accumulation and subsequent liberation the Chennai city. After acclimatization 60 of organic reserves in the hepatopancreas. An eyestalk-ablation study further reveals the Groups (A, B and C) of twenty each. Group degeneration of the hepatopancreas (Kulkarni and Nagabhushanam, 1979). Meera et al., biochemical content on the first day itself.
maturation and biochemical deposition due to eyestalk ablation and fluoxetine treatment concurrently with Group C. Group B received in a brackishwater crab, Uca lactea physiological saline alone at a dose of 25 µl annulipes. From the literature survey it is clear and the Group C received fluoxetine of 25 that mobilization of organic reserves during µl/crab at 1 mM conc. Injections to the two Corresponding Author : M.G. Ragunathan, Department of Advanced Zoology and Biotechnology,
Guru Nanak College, Chennai - 600 042 (India); E-mail : [email protected]
Eswaralakshmi R. et al. (2008) Asian J. Exp. Sci., 22(1); 143-146 Hydroxytrypamine reuptake inhibitor.
shows significant (P<0.01) decrease in sacrificed and the tissues such as haemolymph, compared with the group A further there is taken for biochemical analysis. The different a significant (P<0.01) decrease in the group tissues were stored in icebox until further biochemical analysis of protein carbohydrates standard methods for protein (Lowry et al., shows significant (P<0.01) increase in the 1951), Carbohydrate (Roe, 1955) and lipids (Barnes and Blackstock, 1973). The results obtained from the biochemical analysis were compared with the group B (Table 2).
ovary shows significant (P<0.01) increase group A then there is a significant increase compared with the group A then there is a significant (P<0.01) increase in the group C significant (P<0.01) decrease in the group B when compared with the group A, further, muscle shows significant (P<0.01) decrease there is a significant (P<0.01) decrease in group A and further significant (P<0.01) Table 1 : Total Protein Levels in the Eyestalk Ablated – Fluoxetine treated
Macrobrachium lamarrei lamarrei
(Untreated)
(Saline)
(Fluoxetine)
Each value is mean ± SEM of 10 samples, expressed as mg/gm wet tissue and mg/ml haemolymph.
* P < 0.01 Group A vs B vs C Effects of Eyestalk Ablation and Fluoxetine in a Fresh Water Prawn Table 2 : Total Carbohydrate Levels in the Eyestalk Ablated - Fluoxetine treated
Macrobrachium lamarrei lamarrei
(Untreated)
(Saline)
(Fluoxetine)
Each value is mean ± SEM of 10 samples, expressed as mg/gm wet tissue and mg/ml haemolymph.
* P < 0.01 Group A vs B vs C Table 3 : Total Lipid Levels in the Eyestalk Ablated - Fluoxetine Treated
Macrobrachium lamarrei lamarrei
(Untreated)
(Saline)
(Fluoxetine)
Each value is mean ± SEM of 10 samples, expressed as mg/gm wet tissue and mg/ml haemolymph.
* P < 0.01 Group A vs B vs C Discussion
significant decrease in the carbohydratelevels when compared with their In the present investigation the eyestalk experimental controls. Similarly, ovary and ablated - Fluoxetine treated prawns shows significant decrease in the protein level of Fluoxetine treated group shows significant compared with their experimental controls.
compared with their experimental controls.
Similarly ovary and haemolymph of theeyestalk ablated - Fluoxetine treated group shows significant increase in the protein level ablation accelerated the development offemale gonads, in parallel with when compared with their experimentalcontrols.
transportation of reserves from thehepatopancreas to the ovaries through the haemolymph (Wouters et al., 2001; Arcos et al., 2003; Meera et al., 2006b; Meera ablated - Fluoxetine treated prawns shows Eswaralakshmi R. et al. (2008) Asian J. Exp. Sci., 22(1); 143-146 ablation and gonad development of pacific white shrimp, Litopenaeus vannamei Buone.
sustained activity of gametogenesis during Aquaculture Research, 34, 749-755.
Barnes H. and Blackstock Z.J. (1973): Estimation lamarrei lamarrei was evident from the of lipids in marine animals and tissues.
Detailed investigation of the Phosphovanilin total amount of lipid stored in ovary and method for total lipids. J. Exp. Mar. Bid. hepatopancreas (Kulkarni et al., 2002). In Ecol., 12, 103 – 118.
Kulkarni R.R., Sarojini R. and Nagabhushanam R.
ablated - Fluoxetine treated prawns shows hepatopancreas to ovary during reproductive significant decrease in the lipid level when cycle of fresh water prawn, Macrobrachium compared with their experimental controls.
eyestalk ablated - Fluoxetine treated group Lowry O.H., Rosebrough N.K., Farr A.L. and Randall shows significant increase in the lipid level R.J. (1951). Protein measurement with Folin- phenol reagent. J. Biol. Chem., 193, 265-275.
Meera N., Jayanthi J. and Ragunathan M.G. (2006a): fluoxetine in a brackish water crab, Uca lactea Ragunathan et al. (2007) have reported a annulipes- A Biochemical study. J. Aqua. Biol., significant increase in the organic contents 21(2), 178-179.
Meera N., Jayanthi J. and Ragunathan M.G. (2006b): Nutrient deposition in gonads during their Uca lactea annulipes due to Fluoxetine in maturation due to fluoxetine in an eyestalk ablated fiddler crab, Uca lactea annulipes. J. Aqua. Biol., 21(2), 180-181.
Ragunathan M.G., Meera N., and Jayanthi J. (2007): Prolonged light and fluoxetine stimulates deposition of organic reserves in the gonads fluoxetine treated prawns strongly reveals of a female brackish water crab, Uca lactea annulipes. J. Aqua. Biol., 22 (1), 166-168.
Roe J.R. (1955): The determination of sugar in blood treatment and it is well documented with the and spinal fluid with anthrone reagent. J. Biol. Chem., 20, 335-343.
References
Wouters R., Molina C., Lavens P. and Calderon J.
(2001): Lipid composition and vitamin content Arcos G.F., Ibarra A.M., Boucard C.V., Palacios E.
of the wild female Litopenaeus vannamei.
Different stages of sexual maturation.
metabolic variables in relation to eyestalk Aquaculture, 198, 307-323.

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