Pathology.alfred.org.au

ALFRED HEALTH, Alfred Pathology Service
Alfred Hospital, Caulfield Hospital, Sandringham Hospital
THE COLLECTOR MUST LABEL AND SIGN or INITIAL EVERY SPECIMEN (TUBE) and
MUST COMPLETE THE DECLARATION ON THE REQUEST FORM. Tube Guide for Common Tests
HEPARIN PLASMA with GEL, 5.0 mL
EDTA, 3 mL
• Essential for— FBE, Hb, DCT, Retics Most urgent BIOCHEMISTRY and any
patient on anti-coagulation medication.
• CD4/8, CD2/3, Cell Surface Markers, Flow NOT for Lithium, Holo-TC Vit B
Common
Biochem
• Factor V Leiden, Prothrombin 20210 • ACTH,Ammonia, and Homocysteine on ice.
PLAIN BLOOD (Serum) with GEL, 8.5 mL
• SEROLOGY
(extra tube if more than 4 of these tests)
subclasses, Lithium, Vit D, Methotrexate, Hep A, B, C, HIV, Herpes, Rubella, Syphilis, Autoantibodies, Anticardiolipin Antibodies, GAD, IF, B2GP1, ACL, ENA, MPO, PR3 and Calcitonin, VitA, VitE, C-Peptide all on ice.
(NB: Vit A, Vit C & Vit E - protect from light) FLUORIDE OXALATE, 2 mL
EDTA, 6 mL—‘CROSSMATCH’
• Essential for—
(must be kept at 37°C—call lab first) Remember to date, time and sign TUBE
CITRATE, 2.7 mL (must be complete fill)
EDTA, 10 mL
• COAG—PT, INR, APTT, Fibrinogen,
• Factor VIII, Factor IX, VWF, RCOF, ATIII, Protein C, Protein S, Lupus Screen. CMV viral load, HIV/HBV/HCV Viral Loads, Hep C PCR, Parvovirus PCR and other Viral PCR’s. PLAIN BLOOD (= Serum) without GEL, 10 mL
ACD, 8.5 mL
(must be kept at 37°C—call lab first) see Pathology Handbook for further details • Cytotoxic antibodies, Platelet antibodies • See Next Page (over) for Special Tubes, Paediatric Tubes and Order-of-Draw Chart.
• Tests
printed in Blue & Underlined must be delivered On Ice.
require multiple or separate tubes, consult the Pathology Handbook (intranet) for details.
Warning: Never inject blood into the tube from needle and syringe.
CD_SP_0233_Tube Guide for Common Tests V1.3.doc ALFRED HEALTH, Alfred Pathology Service
Alfred Hospital, Caulfield Hospital, Sandringham Hospital
THE COLLECTOR MUST LABEL AND SIGN or INITIAL EVERY SPECIMEN (TUBE) and
MUST COMPLETE THE DECLARATION ON THE REQUEST FORM. METAL-FREE EDTA, 6 mL
SPECIAL TUBE& (Special Fill Citrate)
PAEDIATRIC (MICRO) COLLECTION CONTAINERS
Tests: as per Tube Guide on page 1
SPECIMEN
PLAIN BLOOD (= Serum) MICROTAINER
with GEL, 600 μL
HEPARIN PLASMA
with GEL, 600 μL
CITRATE, 1.8 mL
CITRATE 1.0 mL
(NOT finger-prick for
ORDER OF DRAW CHART
* Coagulation Tests—When collection is by vacuum tube the coagulation tube should be collected second to avoid artefactual shortening
of coagulation assays due to trauma of venesection. If no other tube is required then collect two blue-top tubes and discard the first.
When blood is collected from an arterial line discard the first 20 mL of blood to clear any heparin from the line before collecting the
blood for coagulation tests.
&ESR—In accordance with NCCLS1 recommendations, ESR tubes should be placed at position 3 above. However, because their position
in the order is not as critical as for citrate specimens for coagulation testing, it is common practice to place this tube at the end of the list
to provide greater priority to tubes that will generally be required for more clinically important tests.
NCCLS document H3-A5 “Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture”. 5th Edition, Dec. 2003. CD_SP_0233_Tube Guide for Common Tests V1.3.doc

Source: http://pathology.alfred.org.au/wp-content/uploads/health-professionals/CD_SP_0233_Tube-Guide-for-Common-Tests-V1.3.pdf