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DATA SHEET
LRP / MVP (Major Vault Protein) Ab-2 (Clone 1032)
Mouse Monoclonal Antibody
Cat. #MS-664-P0, -P1, or -P (0.1ml, 0.5ml, or 1.0ml at 200
µg/ml) (Purified Ab with BSA and Azide)
Cat. #MS-664-P1ABX or -PABX (0.1ml or 0.2ml at 1.0mg/ml)
(Purified Ab without BSA and Azide)
Cat. #MS-664-B0, -B1, or -B (0.1ml, 0.5ml, or 1.0ml at 200
µg/ml) (Biotin-Labeled Ab with BSA and Azide)
Cat. #MS-664-R7 (7.0ml) (Ready-to-Use for Immunohistochemical Staining)
Cat. #MS-664-PCS (5 Slides)
(Positive Control for Histology)
Cat. #MS-664-PCL (0.1ml) (Positive Control for Western Blot)
Description: Major vault protein (MVP) is identical to lung-
Supplied As:
resistance related protein (LRP). . Its shape is reminiscent of the 200µg/ml antibody purified from the ascites fluid by Protein G nucleopore central plug. Treatment of cells with estradiol chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA increases the amount of MVP in nuclear extract.1 The hormone- and 0.09% sodium azide. Also available without BSA and azide at dependent interaction of vaults with ER is prevented in vitro by sodium molybdate.1 Antibodies to progesterone and glucocorticoid Prediluted antibody which is ready-to-use for staining of formalin- receptors are also able to co-immunoprecipitate the MVP.1 Reportedly, the lung-resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and Storage and Stability:
cell lines indicating a poor response to chemotherapy. Ab with sodium azide is stable for 24 months when
Mol. Wt. of Antigen: 104kDa-110kDa
stored at 2-8°C. Antibody WITHOUT sodium azide is stable for 36 months when stored at below 0°C. Epitope: Not determined
Key References:
Species Reactivity: Human and Rat. Others-not
1. Abbondanza C, et al. J Cell Biol 1998;141(6):1301-1310.
Limitations and Warranty:
Clone Designation: 1032
Our products are intended FOR RESEARCH USE ONLY and are not approved for clinical diagnosis, drug use or therapeutic Ig Isotype / Light Chain: IgG1 / κ
procedures. No products are to be construed as a recommendation for use in violation of any patents. We make no representations, Immunogen: Proteins precipitated with AER317
warranties or assurances as to the accuracy or completeness of from the phosphocellulose column flow through of information provided on our data sheets and website. Our warranty extract of human breast cancer MCF-7 cells. is limited to the actual price paid for the product. NeoMarkers is not liable for any property damage, personal injury, time or effort
Applications and Suggested Dilutions:
or economic loss caused by our products. • Gel Supershift Assay (Order Ab at 1mg/ml) Material Safety Data:
This product is not licensed or approved for administration to • Immunoprecipitation (Native verified) humans or to animals other than the experimental animals. Standard Laboratory Practices should be followed when handling (Use Protein G) (Ab 2µg/mg protein lysate) this material. The chemical, physical, and toxicological properties • Western Blotting (Ab 1-2µg/ml for 2hrs at RT) of this material have not been thoroughly investigated. Appropriate measures should be taken to avoid skin and eye contact, inhalation, and ingestion. The material contains 0.09% sodium azide as a Use Ab at 1:50 - 100 for 20 minutes at RT using preservative. Although the quantity of azide is very small, the LP system, for 30 minutes at RT using the appropriate care should be taken when handling this material as UltraVision or UltraVision ONE detection indicated above. The National Institute of Occupational Safety and Health has issued a bulletin citing the potential explosion hazard due to the reaction of sodium azide with copper, lead, brass, or solder in the plumbing systems. Sodium azide forms hydrazoic tissue sections in 10mM citrate buffer, pH 6.0, acid in acidic conditions and should be discarded in a large volume (NEOMARKERS’ Cat. #AP-9003), for 10-20 min
of running water to avoid deposits forming in metal drainage pipes. The optimal dilution for a specific application should For Research Use Only

Positive Control: SKBR-3 cells. Breast tumors.
Cellular Localization: Cytoplasmic

Source: http://www.immunologic.nl/afbeeldingen/bestanden/MS-664-XXX.pdf

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