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Title : analysis of rosiglitazone in rabbit plasma by hptlc


Title : Analysis of Rosiglitazone in rabbit plasma by HPTLC
Author(s) : *S. N. Meyyanathan, Bharani Pandilla, P. Ashok and B. Suresh
*E Mail: meyys@rediffmail.com

ABSTRACT
:
A new simple, precise, rapid, and selective high-performance thin layer chromatography (HPTLC) method was developed for the analysis of Rosiglitazone in rabbit plasma. 2 mg and 4 mg of Rosiglitazone are commercially available as tablets. Only HPLC methods have been reported for the above mentioned drug in the literature and no HPTLC method is available for the estimation in rabbit plasma. The method uses Losartan potassium as an internal standard. The stationary phase used is RP-18 F254s HPTLC plate, prewashed with methanol. The mobile phase consists of methanol : water (10.0 : 2.0 , v/v). Detection and quantification are performed densitometrically at λ = 236 nm. The Rf values of Rosiglitazone and Losartan potassium (IS) are 0.57, and 0.78, respectively. The limits of detection of nimesulide and chlorzoxazone are 0.02 and 0.06 μg respectively. Linearity range for Rosiglitazone is 0.2 - 1.0 μg. Healthy over night fasted animals were used for the experiments. Zero hour fasting blood samples were with drawn early in the morning. The animals were then divided into 3 groups of 6 animals each. The dose for the rabbits was selected based on the surface area ratio of rabbit and man. Group I received 0.3% carboxymethyl cellulose as solvent control. Group 2 received rosiglitazone pure drug and after a wash out period of 15 days group 1 and 2 received the pharmaceutical preparations respectively. The pure drug and the pharmaceutical preparation were suspended in 0.3% carboxy methyl cellulose and the homogenous microsuspensions were administered orally. Immediately after administration the animals were given 5ml of water. Blood samples (1ml each) were with drawn from the marginal ear vein at intervals of 0.5, 1, 2, 3, 4, 6, 8 and 12 hours period using a sterilized syringe. The blood samples collected in the centrifuge tube containing the anti coagulant (100 μl of 11% sodium citrate) were centrifuged at 3000 rpm for 5 minutes and the plasma samples were separated and stored at –20oC. The plasma samples were deproteinised by mixing the sample with equal volume of acetonitrile and the contents were shaken well and allowed to stand for 5 minutes. It was then centrifuged at 3000 rpm for 5 minutes and the supernatant liquid was separated and analysed. Estimation of the plasma samples by HPTLC was carried out using optimized chromatographic conditions. The standard and sample solutions (20 μl) were spotted and the chromatograms were recorded. The plasma drug concentration of the pure drug and pharmaceutical preparations at different time intervals were calculated. Pharmacokinetic parameters such as maximum blood level drug concentration (Cmax), time to peak concentration (Tmax), area under the plasma concentration- time curve (AUC0-12) were calculated. The comparative pharmacokinetic parameters of pure drug and pharmaceutical preparations were calculated. When compared to pure drug, the pharmaceutical preparations shows better bioavailability which is indicated by increased AUC because of the presence of formulation additives. The developed method was suitably validated. The simplicity, accuracy, sensitivity and precision of the developed method makes it as choice for routine analysis of Rosiglitazone in rabbit plasma. Comparative pharmacokinetic parameters of pure drug
and pharmaceutical preparations
Pharmaco
Pure drug
Pharmaceutical preparations
2mg 4mg 2mg
parameters
Typical chromatogram of Rosiglitazone in rabbit plasma

Source: http://www.hptlc.com/pdf/2006pdf/abstract/HPTLC_Rosiglitazone%20in%20rabbit%20plasma_Meyyanathan.pdf

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