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Research Journal Of BioTechnology
Vol. 3 (3) Aug. (2008)
Short Communication
Antimicrobial screening of Andrographis paniculata
(Acanthaceae) root extracts
Radhika P.1*, Sastry B.S.2 and Harika B. Madhu2
1. Department of Biochemistry, Andhra University, Visakhapatnam, (A.P.) 530 003, INDIA.
2. Pharmaceutical Chemistry Division, University College of Pharmaceutical Sciences, Andhra University, Abstract
concentrations of test compound i.e. 100 mg/ml and 200 mg/ml solutions of the extracts. The plates thus prepared were In vitro antibacterial and antifungal activity left for 90 minutes in a refrigerator for diffusion. After of hexane and methanolic extracts of the roots of incubation for 16 hours at 37 0C, the plates were examined Andrographis paniculata were tested against both for inhibition zones. The experiments were performed in gram positive and gram negative bacteria and fungi duplicate and the average diameter of the zones of inhibition by using different techniques. The extracts were measured is recorded. The results are presented in found to inhibit the growth of all the bacteria and fungal organisms tested. The effect produced bythese extracts were comparable with the standard Antifungal activity: Potato dextrose agar (Hi-media) was
antibacterial agent, benzyl penicillin and with the dissolved and distributed in 25 ml quantities in 100 ml conical standard antifungal agent, fluconazole and found flasks and were sterilized in an autoclave at 121 °C (15 lbs/ to be active against all the organisms tested. sq.in) for 20 minutes. The medium was inoculated at onepercent level using 48 hrs old cultures of the test organism Keywords: Andrographis paniculata, hexane extract,
mentioned above aseptically into sterile petri dishes and methanolic extract, antibacterial activity, benzyl penicillin, allowed to set at room temperature for about 30 min. In a size of 4 inches petri dishes, four cups of 8mm diameter at equaldistance were made in each plate. In each plate, one cup was Introduction
used for control i.e. chloroform, other for standard Andrographis paniculata (Family: Acanthaceae) fluconazole with 100 µg/ml, and the other two cups with commonly known as “king of bitters” is a potent stimulator concentrations of test compound i.e.100 mg/ml and 200 mg/ of the immune system, making it effective against a variety ml solutions. The plates thus prepared were left for 90 of infections and oncogenic agents1, anti-viral2, common minutes in a refrigerator for diffusion. After incubation for cold3, fever4, inflammation, antibacterial, antimalarial5, 72 hours at 29 °C, the plates were examined for inhibition filaricidal, antidiarrheal6, antifertility7 and other diseases. In zones .The experiments were performed in duplicate and the view of its diversity of activities the efficacy of the average diameter of the zones of inhibition measured is A. paniculata roots of hexane and methanolic extracts were recorded. The results are presented in Table 2.
screened for their antibacterial activity against bacteria andantifungal activity against fungi by cup diffusion method8 The hexane and methanolic extracts showed less and the results are presented in this communication.
antibacterial activity compared to that of the standard benzylpenicillin, but significant activity was found against both Antibacterial activity: Nutrient agar (Hi-media) was
gram positive and gram negative bacteria. The extracts also dissolved and distributed in 25 ml quantities in 100 ml conical showed significant antifungal activity against fungi but flasks and were sterilized in an autoclave at 121 0C (15lbs/ showed less antifungal activity compared to that of standard sq.in) for 20 minutes. The medium was inoculated at one percent level using 18hrs old cultures of the test organismmentioned above aseptically into sterile petri dishes and Acknowledgement
allowed to set at room temperature for about 30 minutes. In a Financial support from Department of Science and size of 4 inches petri dishes, four cups of 8 mm diameter at Technology (DST), New Delhi under Women Scientists equal distance were made in each plate. In each plate, one Scheme (WOS-A) to Dr. (Mrs.) P. Radhika, Principal cup was used for control i.e. chloroform, other for standard Investigator is gratefully acknowledged.
benzyl penicillin with 100µg/ml, and other two cups with Research Journal Of BioTechnology
Vol. 3 (3) Aug. (2008)
Antibacterial activity of hexane and methanolic root extracts of A. paniculata
Inhibition zone diameter (mm)
Hexane extract
Methanolic extract
Standard
Test organisms
(Benzyl penicillin)
100mg/ml
200mg/ml
100mg/ml
200mg/ml
100ìg/ml
Antifungal activity of hexane and methanolic root extracts of A. paniculata
Inhibition zone diameter (mm)
Standard
Hexane extract
Methanolic extract
Test organisms
(fluconazole)
100mg/ml
200mg/ml
100mg/ml
200mg/ml
100ìg/ml
References
Tandon J.S., Antimalarial activity of Andrographis 1. Puri A., Saxena R., Saxena R.P. and Saxena K.C., paniculata (Kalmegh) against Plasmodim berghei NK 65 in Immunostimulant agents from Andrographis paniculata., Mastomys natalensis. Int. J. Pharmacog. 30(4), 263-274
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2. Weibo L., Prospect for study on treatment of AIDS with 6. Gupta S., Choudhry M.A., Yadava J.N.S., Srivastava V., traditional Chinese medicine. J Trad Chinese Med, 15(1),
and Tandon J.S., Antidiarrheal activity of diterpenes of 3-9 (1995)
Andrographis paniculata (Kalmegh) against Escherichiacoli enterotoxin in in vivo models. Int J Crude Drug Res., 3. Caceres D.D., Hancke J.L., Burgos R.A. and Wikman G.K., 28(4), 273-283 (1990)
Prevention of common colds with Andrographis paniculata
dried extract: A pilot double-blind trial, Phytomedicine, 4(2),
7. Akbarsha M.A., Manivanan B., Hamid K.S. and Vijayan 101-104 (1997)
B., Antifertility effect of Andrographis paniculata (Nees) inmale albino rat., Indian Journal of Experimental Biology, 4. Deng W.L., Preliminary studies on the pharmacology of 28, 421-426 (1990)
the Andrographis product dihydroandrographolide sodium
succinate, Newsletters of Chinese Herbal Med, 8, 26-28
8. Kavanagh E., Analytical Microbiology, Academic Press, New York, 249 (1963).
5. Misra P., Pal N.L., Guru P.Y., Katiyar J.C., Srivastava V. and (Received 2nd April 2008, accepted 8th June 2008) Our journal “Research Journal of BioTechnology” ( ISSN No. 0973-6263 Govt. of India Registration No. MPENG/
2006/18426) is indexed and abstracted in

Chemical Abstracts
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Journal of BioTechnology, Sector AG/80, Scheme 54, A.B. Road, INDORE. Printed at Naidunia Printery,
INDORE. Editor-in-Chief (Honorary) - Dr. S.L. Gargh

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