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Microsoft word - cell line reference list.doc

Published Cell lines used in perfusion cultures with the BioSep, Acoustic cell
retention device.

Cell line
Viable Cell
Aver. conc
density (c/ml)
derived CHO (6)
Human (27)
recombinant CHO
High Five Cells (9)
TB/C3 Mouse (1)
mouse Hybridoma
Mouse mouse
Hybridoma (8)
Hybridoma (23)
Mouse human (30)
human hybridoma
Hybridoma 2E11
Rat Hybridoma
TFL-P (-)
Monodus subeterraneus
UTEX 151 (algae) (2)
Erythraea (fungi) (13)
Sf9 ATCC 1711-
References BioSep
1. H. Bierau, A. Perani, M. Al-Rubeai, A.N. Emery. A comparison of intensive cell culture bioreactors operating with Hybridomas modified for inhibited apoptotic response. Journal ofbiotechnology 62, 195-207, 1998.
2. R. Bosma, W.A. Spronsen, J. Tramper, R. Wijffels. Ultrasound, a new separation technique to harvest microalgae. Journal of Applied Phycology 15, 143-153, 2003.
3. J. Crowley. Using sound waves for cGMP manufacturing of a fusion protein with mammalian cells. Bioprocess International. 46-49, 2004 4. M.C.F. Dalm, S.M.R. Cuijten, W.M.J. van Grunsven, J. Tramper, D.E. Martens. Effect of feed and bleed rate on hybridoma cells in an acoustic perfusion bioreactor: part I. Celldensity, viability and cell-cycle distribution. Biotechnology and bioengineering, Vol. 88 no.5,2004.
5. M.C.F. Dalm, M. Jansen, T.M.P. Keijzer, W.M.J. van Grunsven, A. Oudshoorn, J. Tramper, D.E. Martens. Stable hybridoma cultivation in a pilot scale acoustic perfusion system: Longterm process performance and effect of recirculation rate. Biotechnology and bioengineering,Vol. 91 no.7, 2005.
6. J.E. Dowd, K.E. Kwok, J.M. Piret. Glucose-based optimization of CHO-cell perfusion cultures. Biotechnology and Bioengineering. vol. 75, No. 2. 252-256, 2001.
7. V.M. Gorenflo, L. Smith, B. Dedinsky, B. Person, J. Piret. Scale-up and optimization of an acoustic filter for 200 L/day perfusion of a 100L CHO Cell culture. Biotechnology andBioengineering, Vol 80, no 4, 438-444, 2002.
8. H. Heine, C.Y. Arod, A.R. Bernard, H.D. Blasey, Ultrasonic Cell Separation – Production of Monoclonal Antibodies in Continuous Perfusion Cultures. Poster, 2000.
9. O. Merten. Constructive improvement of the ultrasonic separation device ADI 1015.
10. A.O.A. Miller. Combining cell culture & process operations. Sonoperfusion allows direct feed with expanded-bed chromatography. Genetic engineering news 21, 2001.
11. T. Ryll, G. Dutina, A. Reyes, J. Gunson, L. Krummen, T. Etcheverry, Performance of small- scale CHO perfusion cultures using an acoustic cell filtration device for cell retention:Characterization of separation efficiency and impact of perfusion on product quality.
Biotechnology and Bioengineering, vol. 69 no. 4, 2000.
12. I.Z. Shirgaonkar, S. Lanthier, A. Kamen, Acoustic cell filter: a proven cell retention technology for perfusion of animal cell cultures. Biotechnology advances 22, 433-444, 2004.
13. J.N. Wardell, M.E. Bushell. Kinetics and manipulation of hyphal breakage and its effect on antibiotic production. Enzyme and microbial technology 25, 404-410, 1999.
14. S.M.Woodside, B.D. Bowen, J.M. Piret. Mammalian cell retention devices for stirred perfusion bioreactor. Cytotechnology 28, 163-175, 1998.
15. J. Zhang, A. Collins, M. Chen, I Knyazev, R. Gentz. High-Density Perfusion Culture of Insect Cells with a BioSep Ultrasonic Filter. Biotechnology an Bioengineering, Vol. 59, No.
3, 1998.
18th Esact, 2003.
16. J. Crowley, C. Schlukebir, M. Dijkstal, S. Hoekema, E. Olthof, S. Hussmann, S. Esser, A.
Herman, J.M. Coco Martin, R. Hof. CGMP manufacturing of a fusion protein in mammaliancells using a large scale acoustic perfusion system. Poster 17. M.C.F. Dalm, S.M.R. Cuijten, W.M.J. van Grunsven, A. Oudshoorn, J. Tramper, D.E.
Martens. Growth, death an lysis rate are only slightly influenced by perfusion an bleed rate inperfusion reactor. Poster 18. N. Chatzissavidou, P. Lindberg, H. Petterson, C. Ljung. Performance of CHO perfusion cultures at high cell denisties using acoustic settlers and an improved protein free mediumcomposition. Poster Cell culture engineering VIII. 200219. V.M. Gorenflo, L. Smith, B. Dedinsky, B. Person, J. Piret. Optimization of large scale ultrasonic filter performance for a 100L CHO cell perfusion bioreactor. Poster.
20. V.M. Gorenflo, B.D. Boden, S. Angepat, J. Piret. Optimization of an acoustic perfusion culture with novel air back flush system. Poster.
21. T. Bjorling, V. Chotteau, A. Gretander, O. Tuvesson, U. Dudel. Evaluation of cell separation devices for the perfusion of animal cell culture for biopharmaceutical processes. Poster 22. J. Crowley, C. Schulkebir, M. Dijkstal, S. Hoeksema, E. Olthof, S. Hussman, A. Herman, J.M. Coco Martin. Amino acid metabolism and productivity of CHO in a homogenouscontinuous perfusion culture using a ultrasonic retention device in varied controlledconditions. Poster.
17th Esact, 2001.
23. H.B.A. Wegkamp, N.H. Simpson, B.A. Bulthuis, A.D. Siemensma, D.E. Martens.
Performance of plant hydrolysate in a hybridoma perfusion system. Poster.
24. M. Dűrrschmid, K. Landauer, G. Simic, H. Klug, D. Mueller, T. Keijzer, F. Trampler, A.
Oudshoorn, M. Groschl, O. Doblhoff-Dier. Comparison of fluidised bed and ultrasonic cellretention systems for high cell density mammalian cell culture. Poster.
Other25. Upstream process development – manufacture of recombinant proteins in baby hamster 26. N.A. Kitchen, P.J. Phillips, W. Pilbrough, P.P. Gray. The use of on-line turbidity measurement and an acoustic cell recycle system for perfusion culture of a suspension CHOcell line producing a human therapeutic protein. ECB Spain 2001.
University of Agriculture, IAM, (Prof. H. Katinger)
27. D. Muller, G. Simic, W. Steinfellner, T. Keijzer, O. Doblhoff-Dier, H. Katinger. Continuous perfusion versus discontinuous fed-batch – Production of a cytotoxic glycoprotein in protein-free CHO suspension cultures. Animal cell technology: From target to market. Proceedings ofthe 17th ESACT meeting. 2001.
28. Th. Gaida, O. Doblhoff, K. Strutzenberger, H. Katinger. W Burger, M Groschl, B. Handle, E.
Benes. Scale up of resonance field cell separation devices used in animal cell technology.
poster and internet 29. S. Sonderhoff. Perfusion Culture utilizing acoustic resonance to separate and recycle cells.
30. Doblhoff-Dier, Th. Gaida, H. Katinger, W Burger, M Groschl, E. Benes. A novel Ultrasonic resonance field device for the retention of animal cells. Biotechnology Prog. 10, 1994.
31. Th.Gaida, O. Doblhoff-Dier, K. Strutzenberger, H. Katinger, W Burger, M Groschl, E.
Benes. Selective retention of viable cells in ultrasonic resonance field devices. BiotechnologyProg 12, 73-76, 1996.
32. F. Trampler, S. A. Sonderhoff, P.W.S. Pui, D.G. Kilburn, J.M. Piret. Acoustic cell filter for high density perfusion culture of Hybridoma cells. Biotechnology, 12, 281-284, 1994.
33. P.W.S. Pui, F. Trampler, S. A. Sonderhoff, M. Groeschl, D.G. Kilburn, J.M. Piret. Batch and semicontinuous aggregation and sedimentation of hybridoma cells by acoustic resonancefields. Biotechnology Prog 11, 146-151, 1995.
34. E Benes, F. Hager, W. Bolek, M. Groschl. Separation of dispersed particles by drifting ultrasonic resonance fields. Proceedings of the ultrasonics international 91 conference. 1991 35. C Gatot V. Degouys A.O.A. Miller. High-density cultures of mammalian cells in perfused Cardiff University, (Prof. T. Coakley)
36. W.T. Coakley, D.W. Bardsley, M.A. Grundy. F. Zamani & D.J. Clarke. Cell Manipulation in ultrasonic standing wave field. J. Chem. Tech. Biotechnol. 1989, vol 44. 43-62 37. G. Withworth, M.A. Grundy and W.T. Coakley. Transport and harvesting of suspended particles using modulated ultrsound. Ultrasonics 29, 439-444, 1991.
38. D.G. Kilburn, D.J. Clarke, W.T. Coakley D.W. Bardsley. Enhanced sedimentation of mammalian cells following acoustic aggregation. Biotechnology and bioengineering 559-562.
39. R.K. Gould, W.T. Coakley, M.A. Grundy. Upper sound pressure limits on particle concentration in fields of ultrasonic standing-wave at megahertz frequencies. Ultrasonic 30,239-244, 1992.
40. G. Whitworth W.T. Coakley. Particle column formation in a stationary ultrasonic field.
Journal of acoustic soc. Am. 91, 79-85, 1992.
41. W.T. Coakley, G. Withworth, M.A. Grundy, R.K. Gould, R Allman. Ultrasonic manipulation of particles and cells, ultrasonic separation of cells. Bioseparation 4, 73-83, 1994.
42. R. Allman, W.T. Coakley. Ultrasound enhanced phase partition of microorganisms.
43. M.S. Limaye W.T. Coakley. Clarification of small volume microbial suspension in an ultrasonic standing wave. Society for applied microbiology. 84, 1035-1042, 1998 44. J.J. Hawkes W.T. coakley. A continuous flow ultrasonic cell-filtering method. Enzyme and microbial technology 19, 57-62, 1996.
45. M.S. Limaye, P. Jenkins, J.J. Hawkes, W.T. Coakley. Separation of microorganisms by ultrasonic standing waves. World congress on ultrasonics, 753-756, 1995.
46. M.S. Limaye, J.J. Hawkes. W.T. Coakley. Ultrasonic standing wave removal of microorganisms from suspension in small batch systems. Journal of microbiological methods27, 211-220, 1996.
47. J.J. Hawkes, M.S. Limaye, W.T. Coakley. Filtration of bacteria and yeast by ultrasound- enhanced sedimentation. Society for applied bacteriology, 1996.
48. J.J. Hawkes, D. Barrow, W.T. Coakley. Microparticle manipulation in millimetre scale ultrasonic standing wave chambers. Ultrasonics 36, 925-931, 1998.
49. G. Haar, S.J. Wyard. Blood cells banding in ultrasonic standing wave fields: A physical analysis. Ultrasound in Med & Biol. 4, 111-123, 1978.
50. J.J. Hawkes, T.W. Coakley, Force field particle filter, combining ultrasound standing waves and laminar flow. Sensors and Actuators B, 75, 213-222, 2001.
51. L. Gherardini, J.J. Hawkes, S. Radel, D Mc loughlin W.T. Coakley, M. Groeshl, A.J.
McLoughlin. Micro-manipulation of particles in gel suspension by a cylindrical ultrasonicfield.
52. STS 90, BioSep: a novel separation technology 53. Berg, H. Oudshoorn, A. Trampler, F. Keijzer, T. High density cell cultures in perfusions. A perspective for vaccine production? Bioforum 2001.
54. T. Keijzer, F. Trampler, A. Oudshoorn, O. Doblhoff, H. Berg, Integrating acoustic perfusion in mammalian cell culture. Scale-up and performance characterisation. 2001 (poster) 55. T. Keijzer, F. Trampler, A. Oudshoorn, H. Berg, Integrating acoustic perfusion in mammalian cell culture. Scale-up to production. 2002 (poster) 56. T. Keijzer, F. Trampler, A. Oudshoorn, H. Berg, Integrating acoustic perfusion in mammalian cell culture. Temperature control. 2003 (poster) 57. T. Keijzer. Characterisation of the 10L, 50L and 250L Biosep. Internal report AppliSens, 58. T. Keijzer. Testing steel cuvette 250L biosep, sterilization and separation efficiency. Internal 59. T. Keijzer. Water bath setting of 250L Biosep. Internal report AppliSens, 2000.
60. T. Keijzer. BioSep 1000L. Testing prototype nr 0. Flow distribution and separation. Internal 61. T. Keijzer. Characterization of the APS 990, 10 and 50L controller. Internal report 62. T. Keijzer. Temperature control. Internal report AppliSens, 2003.


Trockene augen neue therapieformen

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